Deleted
Sample Discontinued
Experience: None
Not Used
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Part:BBa_K1773014

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-08-23)


Cas3 protein with pLuxcI right promoter and medium RBS.

This biobrick is composed of I-F type Cas3 helicase-nuclease gene with an pLuxcI right promoter and medium RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2723
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1444
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2407
    Illegal AgeI site found at 2780
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
None